This here presented thesis describes various state of the art identification techniques of fungi diagnostics. The classical microscopic identification procedures are very important in routine diagnosis – they allow a first detection and rough identification of causative pathogen, resulting in a wide range of therapy possibilities for the patient. Moreover, the culture media has a significant impact on the growth behaviour of the fungi; the different media often allow an identification of the various fungi to genus and/or species level. By using this technique, it is possible to ensure a suspicious diagnosis, as well as to conduct an in vitro resistance testing of the fungal pathogen. Concerning the methods for antigen detection, it is necessary to mention the Galactomannan-test as a broadly applied screening technique for the fast detection of the Aspergillus in blood streami.
An important new field in mold diagnostics is molecular diagnosis, which is becoming more and more important since the last few years. Research results demonstrate that with the help of various PCR-based techniques (polymerase chain reaction), for example the Multiplex-PCR or the Real-time-PCR, it is possible to detect very small amounts of fungal DNA (desoxyribonucleinacid) in patient samples.