The species Listeria monocytogenes could not be classified into its serogroups through testing resistances to arsenic, cadmium and benzalkonium chloride by agardilution. Therefore, this method could be hardly used as a further meaningful subtyping method, though the majority of each serotype shows specific patterns of resistances: Isolates of serogroup 1/2a, 3a hardly show resistances to arsenic and are marked by sensitivity as well as resistance to cadmium. Serotype 1/2b, 3b, 7 is sensitive to arsenic, but has increased resistances to cadmium. Strains of serotype 4b, 4d, 4e show the most significant resistances to arsenic and possess partly resistances to cadmium. Resistances to benzalkonium chloride have not been detected. Moreover, strains in conjunction with food-borne and environmental isolates as well as bacterial strains from Upper Austria are sensitive to arsenic and cadmium. As a result, this method could be used to control and follow the spreading and changes of resistances between L. monocytogenes strains.