This thesis deals with the two pathogenic yeast species Candida dubliniensis and Candida albicans, which are phylogenetic closely related, so that it makes it hard to differentiate them. Although they share a wide range of similarities, there are phenotypical as well as biochemical and genetic differences, which can be used for identification. Currently used identification methods depend on growth temperature, carbon source assimilation, chlamydospore and hyphal growth production as well as on intracellular enzymes. However, these methods sometimes fail to recognize those species as they are imprecise. PCR-Assays and DNA-Sequencing are often used for identification, because they have a high sensitivity for detection of those two yeast species. However, most routine laboratories lack of the required equipment. Genetic tests as the sequence analysis and the restriction fragment length polymorphism (RFLP) are also known to give excellent results, but are really costly and time-consuming.