This work deals with a sub-task of a group project. The group project involved the development of a scalable and reproducible two-stage bioprocess, in which Human serum albumin was produced by Pichia pastoris. This work describes the inoculum preparation and inoculation of seed reactor. For this purpose, a cultivation and inoculation system was designed. An R & I diagram of the system and a User requiremts specification with all the necessary equipment was created. The process of inoculum preparation and inoculation of seed reactor were specified in standard operation procedures and master batch records. In addition, the chapter 6 "production" of the Site Master File was created in group work.