Nosocomial infections are infections aquired in hospitals and cause health-deteriorations for the affected persons. They can lead to an extended hospitalisation, a higher mortality and considerable add-on costs. The nursing staff is challenged to prevent these infections, daily. Besides the surveillance of the infection rates, the right and logical conversion of the hygiene regulations is considered the most important preventive measure. Particularly in the contact with central vein catheters, hygienic work is of top priority. A large part of literature’s sources refer to evidence-based hygiene-reccomendations, which are provided by the Centre for Disease Control and Prevention and the Robert-Koch-Institut. It is very important to apply these recommendations strictly, however, a high compliance of the staff is necessary for it. It is possible to reach this by intensive application of trainings, campaigns and care-bundles.