This thesis deals with the interactomic machinery in heterologous protein secreting Pichia pastoris. Here, specific genes were integrated into the genome of this yeast. The idea was, that the additional gene copy number can increase the yield of secreted recombinant proteins. Therefore, the interactomic proteins of secreted model proteins were screened previously and from that study, seven candidates were chosen for cell engineering. The genes were transformed via electroporation, screened and analyzed. The results are presented in this thesis.