Hydrophobic interaction chromatography with monoliths was evaluated as polishing step for a novel live attenuated recombinant lymphocytic choriomeningitis virus vector. Three different kosmotropic salts (ammonium sulfate, trisodium citrate and sodium chloride) were evaluated in different concentrations with respect to virus infectivity, virus quantity and impurity removal using C4 A (butyl) and OH (hydroxyl) ligands. At all tested conditions the viruses bound strongly to the hydrophobic C4 A ligand, but it was not possible to elute viruses efficiently. With the hydrophilic OH ligand the obtained results indicate a stronger dependence of the type of salt and/or the ionic strength. Trisodium citrate showed the best performance and may have the potential to be evaluated further in continuative studies.